Due to the potential for toxicity, solutions containing benzyl alcohol should not be used in neonates. Avoid repeated freeze-thaw cycles: It is essential to avoid repeated freeze-thaw cycles, as they can cause damage to the peptide and decrease its stability and activity. Reactions which may occur because of this solution, added drugs or the technique of reconstitution or administration include febrile response, local tenderness, . Bacteriostatic water vs. sterile water: Reconstitution The stopper is placed on top of the vial and is ultimately seated in the lyophilizer. There have been times when sterile water has been in short supply. Dosage form: injection, solution Do not use unless the solution is clear and seal intact. Parenteral preparations containing benzyl alcohol should not be used in epidural or spinal anesthesia procedures. We focus on finding the most accurate information from the scientific source. Although products may be labeled for reconstitution with Bacteriostatic Water for Injection, Sterile Water for Injection (WFI) should be used to reconstitute products. Validation of these types of filling operations should include media fills and the sampling of critical surfaces and air during active filling (dynamic conditions). They both are nonpyrogenic meaning they wont cause fever. Reconstituted peptides can be stored in several ways, depending on the duration of storage and the desired shelf life. The clinical applications of the hormone hCG include fertility enhancement in both women and men, as well as a supplement to TRT in male subjects [17, 18]. Some drugs can interact poorly with each solvent, and they are not considered universally interchangeable in clinical contexts. (I would personally recommend bacteriostatic water because of its longer shelf-life). You will soon see that your peptide powder will be dissolved and the vial will be transparent. It is not intended nor implied to be a substitute for professional medical advice. How to Use HGH: Mixing, Reconstitution, Injecting Step By Step Guide Experts state that it may be necessary to run trials in order to select the best solvent for various peptides, and sodium chloride water should be avoided [12]. No, you cant just inject bacteriostatic water alone! Peptides the proteins little brother can do wonders for our bodies! Cloudflare Ray ID: 7c0dd62a4baa8226 These Terms will be governed by and interpreted in accordance with the laws of the State of New York, and you submit to the non-exclusive jurisdiction of the state and federal courts located in New York for the resolution of any disputes. 1. : the action of reconstituting or state of being reconstituted. The first step is to use the alcohol swab and wipe the vials containing lyophilized peptides and the bacteriostatic/sterile water. Sterile water is free of any organic materials but contains inorganic materials to some extent. Should I dilute with sterile water (USP grade) or BW? Its preservative content keeps it sterile to prevent infection, and its chemical properties allow it to efficiently dissolve solutes without compromising their integrity. In closing, bacteriostatic water is often the preferred choice for injections in a range of contexts. When it comes to reconstituting peptides, there is a common question of whether bacteriostatic water is the same as sterile water. Reviewed in the United States on January 28, 2023. However, they do differ in their composition as the bacteriostatic water contains 0.9% benzyl alcohol as a preservative. Bacteriostatic Water may be used alone or with other medications. did you ever get an answer? Due to the lack of antimicrobials, sterile water can easily be contaminated once exposed to surroundings and even yourself. From medications to peptides and hormones, bacteriostatic water is a winning option when it comes to reconstitution and sterility. It is supplied in a multiple-dose container from which repeated withdrawals may be made to dilute or dissolve drugs for injection. Parenteral preparations with benzyl alcohol should not be used for fluid replacement. Also, nothing contained on this Website shall be interpreted as advising you. Store at 20 to 25C (68 to 77F). We spent 20 hours of research finding optimal recommended dosages for every popular peptide. Please let me know if you get an answer. Wipe down all materials with an alcohol swab. Two sterile solutions that are commonly used are bacteriostatic water and sterile water. In the event these should occur, re-evaluate the patient and institute appropriate corrective measures. When reconstituted with Bacteriostatic Water for Injection, USP, the resulting solution must be used within 7 days. Administration of an estimated 9 mL to a 6 kg infant or neonate is potentially capable of producing blood pressure changes. Dont waste time and energy attempting to source syringes, alcohol swabs, and bacteriostatic water from multiple retailers. Gently remove the needle from the vial. First, you want to thoroughly wash your hands with soap and water for at least 20 seconds. Bacteriostatic Water I am sure that most of the things would be clear by now. Reconstitution follows the above method. For the reconstitution of HGH powder, bacteriostatic water is a great choice, as it preserves HGH solutions and efficiently dissolves lyophilized aggregates [16]. Only if the peptides are of the same nature such as growth hormone (GH) influencers or if you see any vendors selling a stack of particular peptides, only those you should mix. Reactions which may occur because of this solution, added drugs or the technique of reconstitution or administration include febrile response, local tenderness, abscess, tissue necrosis or infection at the site of injection, venous thrombosis or phlebitis extending from the site of injection and extravasation. IMPORTANT Use only the accompanying diluent or Bacteriostatic Water For Injection with. By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. Bacteriostatic Water for Peptides | Learn How to Prepare Bacteriostatic Lets just assume that you have been prescribed 500mcg of BPC 157. 11, pp. ], Distributed by Hospira, Inc., Lake Forest, IL 60045 USA. Medications that required diluting with water usually indicate on the bottle which water is preferred for use. Insert your needle tip into the sealed bottle of bacteriostatic water. [See USP Controlled Room Temperature. PDF SOLU-MEDROL (methylprednisolone sodium succinate for injection, USP The Bacteriostatic Water is available generically and has the same name. Dont just shoot the water like a spray or something. The second step is to use your syringe and draw out 1ml of bacteriostatic water from the vial without really touching the needle as it could cause contamination. What Is Bacteriostatic Water For Peptide Reconstitution These are the primary similarities between bacteriostatic water and sterile water [5]: Here are the key differences between bacteriostatic water and sterile water [5]: Despite their similarities, bacteriostatic water and sterile water differ in a few key areas. 1) Determine the quantity of peptide, in micrograms (mcg), in your vial. Bacteriostatic Water vs. Sterile Water | Deus Medical These include subcutaneous, intramuscular, intravenous, and intradermal injection routes [. Because they are antibiotics, low bioburden of these formulations would be expected at the time of batching. However, you are not allowed to assign, transfer, or subcontract any of your rights and/or obligations under these Terms. By displaying Your Content, you grant Buckle LLC a non-exclusive, worldwide irrevocable, sub licensable license to use, reproduce, adapt, publish, translate and distribute it in any and all media. Some peptides are still off-limits to us mere mortals, and only available for research purposes. pH of the reconstituted solution is within the USP specified range of 7 to 8 and the tonicities are, for the 40 mg per mL solution, 0.50 osmolar; for the 125 mg per 2 mL solution, 0.40 osmolar; for the 1 gram per 8 mL solution, 0.44 osmolar; for the 2 gram per 30.6 mL solutions, 0.42 osmolar. Bacteriostatic Water (Bacteriostatic Water for Injection - RxList All of our content is written by people with a strong science background, including medical researchers. Your IP: Storing peptides is a very crucial step because a slight fluctuation in the physical conditions can really degrade the peptide vial. It is recognized that there is complex technology associated with the manufacture and control of a lyophilized pharmaceutical dosage form. You should never inject bacteriostatic water without mixing in any medications because the benzyl alcohol in it causes the red blood cell lysis. Bacteriostatic water (BW) has a pH of about 5.7. Helpful. Obviously, there is a much greater chance of contamination in this type of operation because of the involvement of people. Typical finished product testing that should be reviewed includes dose uniformity testing, sterility testing, and any required stability testing of aged batches of reconstituted solutions. Parenteral drug products should be inspected visually for particulate matter Additionally, bacteriostatic water must always be mixed with a solute before injection. Be sure to consult indications for exact dosage and concentration. Always double-check which mixture you have because you cannot interchange between these two formulations. Once vials are partially stoppered, they are transported to the lyophilizer. Also, if you are experimenting with new peptides, it is always best to use bacteriostatic water to dilute the peptides. What is the resulting supply dose in mg/mL? . Administration of an estimated 9 mL to a 6 kg infant or neonate is potentially capable of producing blood pressure changes. Bacteriostatic water (BW) has a pH of about 5.7. If you have an allergy to benzyl alcohol, discontinue or avoid bacteriostatic water and opt for sterile water. The universal rule is to use 1 milliliter of bacteriostatic water for every peptide that you reconstitute. Research peptides present great potential in emerging clinical contexts, such as combating symptoms of cardiovascular and metabolic diseases, as well as improving reproductive health [11]. Bacteriostatic Water for Injection may kill some of the vegetative cells if present as contaminants. Using in such a matter can cause hemolysis or destruction of red blood cells. Although, a small percentage of people report inflammation at the injection site if sterile water is used to dilute peptides that are to be injected intramuscularly. Slowly extract the bacteriostatic water until the solution reaches your desired level. Reconstitution Solution 4-Pack (30mL) Bactostatic Labs. It would seem very difficult for a manufacturer to be able to demonstrate satisfactory validation of sterilization of a lyophilizer by chemical "treatment.". Parenteral preparations with benzyl alcohol should not be used for fluid replacement. It is essential to avoid repeated freeze-thaw cycles and exposure to light and high temperatures. Those are bacteriostatic and sterile water. Because many of the types of products are antibiotics, subpotency in a vial can present a potentially very serious situation. The starter set has all you need to get going, including the following: If you want a little more, check out the premium kit: Qualified handlers are sure to enjoy this top online source for research materials. Additionally, this stability testing should include least concentrated as well as the most concentrated reconstituted solutions. It is essential to avoid exposure to light and high temperatures, which can lead to peptide degradation. You hereby indemnify to the fullest extent Buckle LLC from and against any and/or all liabilities, costs, demands, causes of action, damages and expenses arising in any way related to your breach of any of the provisions of these Terms. Consult the manufacturer's instructions for choice of vehicle, appropriate dilution or volume for dissolving the drugs to be injected, including the route and rate of injection. Specialists generally consider bacteriostatic water to be a more efficient solvent or dilutant. and our RxList does not provide medical advice, diagnosis or treatment. Stability data should be reviewed and the justification of the expiration date should be based on batches with the higher moisture content. Pregnancy: Animal reproduction studies have not been conducted with Bacteriostatic Water for Injection. You don't use either to reconstitute it. Click to reveal Although rarely, but they can occur, those side effects are more related to the drug that is dissolved in the bacteriostatic water. Overall, specialists agree that bacteriostatic water for injection is safer and more effective in most cases. This bacteriostat is usually 0.9% benzyl alcohol. Methylprednisolone sodium succinate has the same . HCG solutions are to be refrigerated to prevent degradation [19]. Read the full review on BPC 157 only at Muscle and Brawn. Long-term storage: For long-term storage, it is recommended to aliquot the reconstituted peptide into small volumes and freeze them at -20C or -80C. If I have a 2mg bottle how much water total do I put into the vial? With our easy-to-use reconstitution calculator, you now dont have to worry about all the multiplication, division or the unit conversion and all. Bacteriostatic water has wider availability and is simpler to manufacture. Privacy Policy. A generally recognized acceptable method of sterilizing the lyophilizer is through the use of moist steam under pressure. For more information, please see our You use AA, wich is a .6% solution of acetic acid. To really know the best solvent in which a peptide will be soluble, you must have to consider some biochemistry knowledge and do some trial and error. For safe storage, adhere to manufacturer indications. Lets explore their points of similarity and difference to understand their appropriate uses. When diluting or dissolving drugs, mix thoroughly and use promptly. BACTERIOSTATIC 0.9% SODIUM CHLORIDE INJECTION, USP 10mL VIAL Unused vials can remain viable for years with proper storage [4]. Do not use unless the solution is clear and seal intact. Daptomycin RF has a longer in-use shelf period although times vary based on dosage form (vial, IV bag, or syringe) and diluent used. As I mentioned at the start, the sterile water vial doesnt contain benzyl alcohol which makes it unfavorable for storage. However, some of the other dosage forms that are lyophilized, such as hydrocortisone sodium succinate and methylprednisolone sodium succinate have no antibacterial effect when in solution. Two independent temperature sensing systems. I need to rehydrate some BPC, but when I look for bacteriostatic water on Amazon, it takes me directly to "reconstitution solution." 51.222.156.250 5.0 out of 5 stars Good product. You'll need an alcohol wipe, your bacteriostatic water and HCG vials, your reconstitution syringe and needle, and a Sharps container for safe disposal. Peptide Reconstitution + Mixing Guide With Calculator - Muscle and Brawn Sample 1 Sample 2 Sample 3. If you feel that any of our content is inaccurate or out-of-date, please contact us at: [emailprotected]. It would ideally be either 0.3ml, 0.5ml or 1ml syringe. The choice is purely up to the user which solution to use. Besides neonates, they both can be used for the rest of the population including pediatrics, adults and the geriatric community. Inspect reconstituted (diluted or dissolved) drugs for clarity (if soluble) and freedom from unexpected precipitation or discoloration prior to administration. .063 mg/mL, 3200 mL c. 1000 mg/mL, 5 mL d. 64 mg/mL b. It is only used for injections such as intravenous, intramuscular and subcutaneous. Do NOT reuse the same saline syringe to flush VADs before and after medication administration. This parenteral preparation is unlikely to You can email the site owner to let them know you were blocked. This website is using a security service to protect itself from online attacks. The concern with any microbiological level is the possible increase in endotoxins that may develop. Handlers should adhere to the exact indications or medical instructions. This material is provided for educational purposes only and is not intended for medical advice, diagnosis or treatment. The small volume of fluid provided by Bacteriostatic Water for Injection, USP when used only as a pharmaceutic aid for diluting or dissolving drugs for parenteral injection, is unlikely to exert a significant effect on fluid balance except possibly in very small infants. Once you have put the details, our reconstitution calculator will use its algorithm and devise the volume needed to get your peptides reconstituted! Before really using the peptide, you will have to add liquid to the powder and MIX it well to make it a solution but it isnt as simple as it sounds! Carefully install the needle tip on a syringe. In case you experience any of these side effects, you should immediately stop using sterile water for injections and talk to a doctor. The Bacteriostatic Water can be used in diluting drugs that can subsequently be administered by intravenous, . That means, they are single-dose vials and should be discarded after a single-use. Although both of these types of water are sterile, the difference between bacteriostatic water and sterile water is that other sterile waters for injection do not contain a bacteriostatic agent. The action you just performed triggered the security solution. But, if there is an absolute need to stock solution form, what I would recommend is to store the required dosage into individual vials. The next step is to do is add the mass of the peptide in mg that you want to reconstitute (This is generally going to be listed on the peptide vial) with the x ml of bacteriostatic water in order to get your desirable dosage in mcg. By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. Bacteriostatic water is a sterile form of water that has been brought to a pH between 4.5 to 7.0. Bacteriostatic Water vs "reconstitution solution" what's - Reddit Both act as dilutants or solvents of substances to enable injection. The small volume of fluid provided by Bacteriostatic Water for Injection, USP when used only as a pharmaceutic aid for diluting or dissolving drugs for parenteral injection, is unlikely to exert a significant effect on fluid balance except possibly in very small infants. This website is using a security service to protect itself from online attacks. This monograph has been modified to include the generic and brand name in many instances. Data sources include IBM Watson Micromedex (updated 1 May 2023), Cerner Multum (updated 17 Apr 2023), ASHP (updated 10 Apr 2023) and others. Validation of this handling should also include the use of filled vials of media. Peptide reconstitution is a complicated and confusing process for many. Bacteriostatic water is contraindicated, or forbidden, from being used in neonates, newborns in the first 28 days of their life. Bacteriostatic Water for Injection: PI - Drugs.com I recommend using bacteriostatic water since that's what the peptide vendors sell and recommend. Bacteriostatic Sodium Chloride: Package Insert - Drugs.com Prior to buying anything, check that it is compliant where you live with your current government laws. How to Properly Mix, Dose, and Store Peptides - Paradigm Peptides AOD 9604 can either be reconstituted in bacteriostatic water (BAC) or sterile water. Why is Water Essential to Reconstitution? Reconstitution means any Securitization Transaction or Whole Loan Transfer. As mentioned previously, sterile water only has distilled water and nothing else. One vial of BPC 157 contains 5mg, which also means 5000mcg. Acidic peptides will be best soluble in basic solution and vice versa. They can manage our circadian rhythms, regulate growth hormones, help with weight loss, and even make us look younger.
